Neuropeptidomics: expanding proteomics downwards.

Neuropeptidomics: mass spectrometry-based qualitative and quantitative analysis.

Neuropeptidomics refers to a global characterization approach for the investigation of neuropeptides, often under specific physiological conditions. Neuropeptides comprise a complex set of signaling molecules that are involved in regulatory functions and behavioral control in the nervous system. Neuropeptidomics is inherently challenging because neuropeptides are spatially, temporally, and chem...

Improved Neuropeptide Identification Bioinformatics and Mass Spectrometry

List of papers This thesis is based on the papers listed below, which are referred to in the text by Roman numerals I-V. Neuropeptidomics: MS applied to the discovery of novel peptides from the brain. An automated method for scanning LC-MS data sets for significant peptides and proteins, including quantitative profiling and interactive confirmation. Striatal proteomic analysis suggests that fir...

Quantitative neuropeptidomics of microwave-irradiated mouse brain and pituitary.

In neuropeptidomics, the degradation of a small fraction of abundant proteins overwhelms the low signals from neuropeptides, and many neuropeptides cannot be detected by mass spectrometry without extensive purification. Protein degradation was prevented when mice were sacrificed with focused microwave irradiation, permitting the detection of hypothalamic neuropeptides by mass spectrometry. Here...

Neuropeptidomics: Ms-base Peptide Identification and Quantitation Study By

Chronic Nicotine Treatment Impacts the Regulation of Opioid and Non-opioid Peptides in the Rat Dorsal Striatum*□S

The chronic use of nicotine, the main psychoactive ingredient of tobacco smoking, alters diverse physiological processes and consequently generates physical dependence. To understand the impact of chronic nicotine on neuropeptides, which are potential molecules associated with dependence, we conducted qualitative and quantitative neuropeptidomics on the rat dorsal striatum, an important brain r...